Construct: shRNA TRCN0000148572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133463.1-2996s1c1
DNA Barcode:
CTGCACTTTGAAGCTCCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMZ1 (155185)
Gene Description:
archaelysin family metallopeptidase 1
Transcript:
RefSeq NM_133463.1 (NON-CURRENT)
Match location:
Position 2996 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCACTTTGAAGCTCCTTTA
Hairpin Sequence:
5'-CCGG-CTGCACTTTGAAGCTCCTTTA-CTCGAG-TAAAGGAGCTTCAAAGTGCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCACTTTGAAGCTCCTTTACTCGAGTAAAGGAGCTTCAAAGTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCACTTTGAAGCTCCTTTACTCGAGTAAAGGAGCTTCAAAGTGCAG-3'

Other clones with same target sequence:

(none)