Construct: shRNA TRCN0000148648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_214461.1-1241s1c1
DNA Barcode:
CCTGCCCAAGGATTTATTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C2orf27B (408029)
Gene Description:
chromosome 2 open reading frame 27B
Transcript:
RefSeq NM_214461.1 (NON-CURRENT)
Match location:
Position 1241 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCCAAGGATTTATTCAT
Hairpin Sequence:
5'-CCGG-CCTGCCCAAGGATTTATTCAT-CTCGAG-ATGAATAAATCCTTGGGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCCAAGGATTTATTCATCTCGAGATGAATAAATCCTTGGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCCAAGGATTTATTCATCTCGAGATGAATAAATCCTTGGGCAGG-3'

Other clones with same target sequence:

(none)