Construct: shRNA TRCN0000148726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018240.3-1626s1c1
DNA Barcode:
CATCTTCTTCTTCATCGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIRREL1 (55243)
Gene Description:
kirre like nephrin family adhesion molecule 1
Transcript:
RefSeq NM_018240.3 (NON-CURRENT)
Match location:
Position 1626 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTTCTTCTTCATCGCCTT
Hairpin Sequence:
5'-CCGG-CATCTTCTTCTTCATCGCCTT-CTCGAG-AAGGCGATGAAGAAGAAGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTTCTTCTTCATCGCCTTCTCGAGAAGGCGATGAAGAAGAAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTTCTTCTTCATCGCCTTCTCGAGAAGGCGATGAAGAAGAAGATG-3'

Other clones with same target sequence:

(none)