Construct: shRNA TRCN0000148740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003752.2-1318s1c1
DNA Barcode:
CCTGTTTGCAAATCCCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF3C (8663)
Gene Description:
eukaryotic translation initiation factor 3 subunit C
Transcript:
RefSeq NM_003752.2 (NON-CURRENT)
Match location:
Position 1318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTTGCAAATCCCAACAT
Hairpin Sequence:
5'-CCGG-CCTGTTTGCAAATCCCAACAT-CTCGAG-ATGTTGGGATTTGCAAACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTTGCAAATCCCAACATCTCGAGATGTTGGGATTTGCAAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTTGCAAATCCCAACATCTCGAGATGTTGGGATTTGCAAACAGG-3'

Other clones with same target sequence:

(none)