Construct: shRNA TRCN0000148938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003028.1-576s1c1
DNA Barcode:
CCAAGTGGCTAAACAAGTACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHB (6461)
Gene Description:
SH2 domain containing adaptor protein B
Transcript:
RefSeq NM_003028.1 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTGGCTAAACAAGTACT
Hairpin Sequence:
5'-CCGG-CCAAGTGGCTAAACAAGTACT-CTCGAG-AGTACTTGTTTAGCCACTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTGGCTAAACAAGTACTCTCGAGAGTACTTGTTTAGCCACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTGGCTAAACAAGTACTCTCGAGAGTACTTGTTTAGCCACTTGG-3'

Other clones with same target sequence:

  1. TRCN0000285376