Construct: shRNA TRCN0000148975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005953.2-119s1c1
DNA Barcode:
GCAAAGAGTGCAAATGCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT2A (4502)
Gene Description:
metallothionein 2A
Transcript:
RefSeq NM_005953.2 (NON-CURRENT)
Match location:
Position 119 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGAGTGCAAATGCACTT
Hairpin Sequence:
5'-CCGG-GCAAAGAGTGCAAATGCACTT-CTCGAG-AAGTGCATTTGCACTCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGAGTGCAAATGCACTTCTCGAGAAGTGCATTTGCACTCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGAGTGCAAATGCACTTCTCGAGAAGTGCATTTGCACTCTTTGC-3'

Other clones with same target sequence:

(none)