Construct: shRNA TRCN0000148977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014869.2-2551s1c1
DNA Barcode:
GAAGAAATTCACCGATGACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IQSEC1 (9922)
Gene Description:
IQ motif and Sec7 domain ArfGEF 1
Transcript:
RefSeq NM_014869.2 (NON-CURRENT)
Match location:
Position 2551 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAATTCACCGATGACCT
Hairpin Sequence:
5'-CCGG-GAAGAAATTCACCGATGACCT-CTCGAG-AGGTCATCGGTGAATTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAATTCACCGATGACCTCTCGAGAGGTCATCGGTGAATTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAATTCACCGATGACCTCTCGAGAGGTCATCGGTGAATTTCTTC-3'

Other clones with same target sequence:

(none)