Construct: shRNA TRCN0000149046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006260.2-919s1c1
DNA Barcode:
GAGCCAAGCATTGCTGAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJC3 (5611)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C3
Transcript:
RefSeq NM_006260.2 (NON-CURRENT)
Match location:
Position 919 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCCAAGCATTGCTGAATAT
Hairpin Sequence:
5'-CCGG-GAGCCAAGCATTGCTGAATAT-CTCGAG-ATATTCAGCAATGCTTGGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCCAAGCATTGCTGAATATCTCGAGATATTCAGCAATGCTTGGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCCAAGCATTGCTGAATATCTCGAGATATTCAGCAATGCTTGGCTC-3'

Other clones with same target sequence:

(none)