Construct: shRNA TRCN0000149061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033514.1-366s1c1
DNA Barcode:
GACTCTTCTATGAGGAACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIMS3 (96626)
Gene Description:
LIM zinc finger domain containing 3
Transcript:
RefSeq NM_033514.1 (NON-CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCTTCTATGAGGAACGAA
Hairpin Sequence:
5'-CCGG-GACTCTTCTATGAGGAACGAA-CTCGAG-TTCGTTCCTCATAGAAGAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCTTCTATGAGGAACGAACTCGAGTTCGTTCCTCATAGAAGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCTTCTATGAGGAACGAACTCGAGTTCGTTCCTCATAGAAGAGTC-3'

Other clones with same target sequence:

(none)