Construct: shRNA TRCN0000149086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020463.1-3887s1c1
DNA Barcode:
GTAATGTGTTACGGGTCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP4R3B (57223)
Gene Description:
protein phosphatase 4 regulatory subunit 3B
Transcript:
RefSeq NM_020463.1 (NON-CURRENT)
Match location:
Position 3887 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAATGTGTTACGGGTCCATT
Hairpin Sequence:
5'-CCGG-GTAATGTGTTACGGGTCCATT-CTCGAG-AATGGACCCGTAACACATTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAATGTGTTACGGGTCCATTCTCGAGAATGGACCCGTAACACATTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAATGTGTTACGGGTCCATTCTCGAGAATGGACCCGTAACACATTAC-3'

Other clones with same target sequence:

(none)