Construct: shRNA TRCN0000149163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024329.4-1996s1c1
DNA Barcode:
GCAACTTTCAGTGTGTGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFHD2 (79180)
Gene Description:
EF-hand domain family member D2
Transcript:
RefSeq NM_024329.4 (NON-CURRENT)
Match location:
Position 1996 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACTTTCAGTGTGTGTCAT
Hairpin Sequence:
5'-CCGG-GCAACTTTCAGTGTGTGTCAT-CTCGAG-ATGACACACACTGAAAGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACTTTCAGTGTGTGTCATCTCGAGATGACACACACTGAAAGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACTTTCAGTGTGTGTCATCTCGAGATGACACACACTGAAAGTTGC-3'

Other clones with same target sequence:

(none)