Construct: shRNA TRCN0000149172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_214462.1-2769s1c1
DNA Barcode:
GTTTCCCAATCCTTTGACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DACT2 (168002)
Gene Description:
dishevelled binding antagonist of beta catenin 2
Transcript:
RefSeq NM_214462.1 (NON-CURRENT)
Match location:
Position 2769 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTCCCAATCCTTTGACCAT
Hairpin Sequence:
5'-CCGG-GTTTCCCAATCCTTTGACCAT-CTCGAG-ATGGTCAAAGGATTGGGAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTCCCAATCCTTTGACCATCTCGAGATGGTCAAAGGATTGGGAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTCCCAATCCTTTGACCATCTCGAGATGGTCAAAGGATTGGGAAAC-3'

Other clones with same target sequence:

(none)