Construct: shRNA TRCN0000149264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207335.1-327s1c1
DNA Barcode:
GCTGTAGTCAACAGTGAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KBTBD12 (166348)
Gene Description:
kelch repeat and BTB domain containing 12
Transcript:
RefSeq NM_207335.1 (NON-CURRENT)
Match location:
Position 327 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTAGTCAACAGTGAGATT
Hairpin Sequence:
5'-CCGG-GCTGTAGTCAACAGTGAGATT-CTCGAG-AATCTCACTGTTGACTACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTAGTCAACAGTGAGATTCTCGAGAATCTCACTGTTGACTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTAGTCAACAGTGAGATTCTCGAGAATCTCACTGTTGACTACAGC-3'

Other clones with same target sequence:

(none)