Construct: shRNA TRCN0000149269

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006335.1-1380s1c1
DNA Barcode:
GCACTTCAGAACAGGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIMM17A (10440)
Gene Description:
translocase of inner mitochondrial membrane 17A
Transcript:
RefSeq NM_006335.1 (NON-CURRENT)
Match location:
Position 1380 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149269)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTCAGAACAGGTCATTT
Hairpin Sequence:
5'-CCGG-GCACTTCAGAACAGGTCATTT-CTCGAG-AAATGACCTGTTCTGAAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTCAGAACAGGTCATTTCTCGAGAAATGACCTGTTCTGAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTCAGAACAGGTCATTTCTCGAGAAATGACCTGTTCTGAAGTGC-3'

Other clones with same target sequence:

(none)