Construct: shRNA TRCN0000149276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138967.2-216s1c1
DNA Barcode:
GCCATGTTTCTACCAAGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCAMP5 (192683)
Gene Description:
secretory carrier membrane protein 5
Transcript:
RefSeq NM_138967.2 (NON-CURRENT)
Match location:
Position 216 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTTTCTACCAAGACTT
Hairpin Sequence:
5'-CCGG-GCCATGTTTCTACCAAGACTT-CTCGAG-AAGTCTTGGTAGAAACATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTTTCTACCAAGACTTCTCGAGAAGTCTTGGTAGAAACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTTTCTACCAAGACTTCTCGAGAAGTCTTGGTAGAAACATGGC-3'

Other clones with same target sequence:

  1. TRCN0000105536
  2. TRCN0000325775