Construct: shRNA TRCN0000149290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014753.2-1028s1c1
DNA Barcode:
GCGCTGTTTAAATGAGAAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMS1 (9790)
Gene Description:
BMS1 ribosome biogenesis factor
Transcript:
RefSeq NM_014753.2 (NON-CURRENT)
Match location:
Position 1028 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTGTTTAAATGAGAAGGA
Hairpin Sequence:
5'-CCGG-GCGCTGTTTAAATGAGAAGGA-CTCGAG-TCCTTCTCATTTAAACAGCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTGTTTAAATGAGAAGGACTCGAGTCCTTCTCATTTAAACAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTGTTTAAATGAGAAGGACTCGAGTCCTTCTCATTTAAACAGCGC-3'

Other clones with same target sequence:

(none)