Construct: shRNA TRCN0000149303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080928.2-1335s1c1
DNA Barcode:
GCATGGTGACATCTTTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASB15 (142685)
Gene Description:
ankyrin repeat and SOCS box containing 15
Transcript:
RefSeq NM_080928.2 (NON-CURRENT)
Match location:
Position 1335 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGTGACATCTTTGGAAA
Hairpin Sequence:
5'-CCGG-GCATGGTGACATCTTTGGAAA-CTCGAG-TTTCCAAAGATGTCACCATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGTGACATCTTTGGAAACTCGAGTTTCCAAAGATGTCACCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGTGACATCTTTGGAAACTCGAGTTTCCAAAGATGTCACCATGC-3'

Other clones with same target sequence:

(none)