Construct: shRNA TRCN0000149347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006317.3-230s1c1
DNA Barcode:
GAAAGCCAAGGAGAAAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BASP1 (10409)
Gene Description:
brain abundant membrane attached signal protein 1
Transcript:
RefSeq NM_006317.3 (NON-CURRENT)
Match location:
Position 230 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGCCAAGGAGAAAGACAA
Hairpin Sequence:
5'-CCGG-GAAAGCCAAGGAGAAAGACAA-CTCGAG-TTGTCTTTCTCCTTGGCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGCCAAGGAGAAAGACAACTCGAGTTGTCTTTCTCCTTGGCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGCCAAGGAGAAAGACAACTCGAGTTGTCTTTCTCCTTGGCTTTC-3'

Other clones with same target sequence:

(none)