Construct: shRNA TRCN0000149363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173532.1-1113s1c1
DNA Barcode:
GCATCACATGTGGCATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FNDC7 (163479)
Gene Description:
fibronectin type III domain containing 7
Transcript:
RefSeq NM_173532.1 (NON-CURRENT)
Match location:
Position 1113 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCACATGTGGCATCAATT
Hairpin Sequence:
5'-CCGG-GCATCACATGTGGCATCAATT-CTCGAG-AATTGATGCCACATGTGATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCACATGTGGCATCAATTCTCGAGAATTGATGCCACATGTGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCACATGTGGCATCAATTCTCGAGAATTGATGCCACATGTGATGC-3'

Other clones with same target sequence:

(none)