Construct: shRNA TRCN0000149464

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001034844.1-318s1c1
DNA Barcode:
GCTTGAGGATCCTGTCATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPDYE7P (441251)
Gene Description:
speedy/RINGO cell cycle regulator family member E7, pseudogene
Transcript:
RefSeq NM_001034844.1 (NON-CURRENT)
Match location:
Position 318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149464)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAGGATCCTGTCATTAA
Hairpin Sequence:
5'-CCGG-GCTTGAGGATCCTGTCATTAA-CTCGAG-TTAATGACAGGATCCTCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAGGATCCTGTCATTAACTCGAGTTAATGACAGGATCCTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAGGATCCTGTCATTAACTCGAGTTAATGACAGGATCCTCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000163014