Construct: shRNA TRCN0000149537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024650.2-1521s1c1
DNA Barcode:
GTATTCTGACTGGAAGCACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C11orf80 (79703)
Gene Description:
chromosome 11 open reading frame 80
Transcript:
RefSeq NM_024650.2 (NON-CURRENT)
Match location:
Position 1521 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTCTGACTGGAAGCACTA
Hairpin Sequence:
5'-CCGG-GTATTCTGACTGGAAGCACTA-CTCGAG-TAGTGCTTCCAGTCAGAATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTCTGACTGGAAGCACTACTCGAGTAGTGCTTCCAGTCAGAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTCTGACTGGAAGCACTACTCGAGTAGTGCTTCCAGTCAGAATAC-3'

Other clones with same target sequence:

(none)