Construct: shRNA TRCN0000149556

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025234.1-868s1c1
DNA Barcode:
GACTTGTGTTCACACCTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR61 (80349)
Gene Description:
WD repeat domain 61
Transcript:
RefSeq NM_025234.1 (NON-CURRENT)
Match location:
Position 868 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149556)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTGTGTTCACACCTTCTT
Hairpin Sequence:
5'-CCGG-GACTTGTGTTCACACCTTCTT-CTCGAG-AAGAAGGTGTGAACACAAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTGTGTTCACACCTTCTTCTCGAGAAGAAGGTGTGAACACAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTGTGTTCACACCTTCTTCTCGAGAAGAAGGTGTGAACACAAGTC-3'

Other clones with same target sequence:

  1. TRCN0000330144