Construct: shRNA TRCN0000149557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025267.2-859s1c1
DNA Barcode:
GAAGACAACATCATGGGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AARSD1 (80755)
Gene Description:
alanyl-tRNA synthetase domain containing 1
Transcript:
RefSeq NM_025267.2 (NON-CURRENT)
Match location:
Position 859 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACAACATCATGGGAGTT
Hairpin Sequence:
5'-CCGG-GAAGACAACATCATGGGAGTT-CTCGAG-AACTCCCATGATGTTGTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACAACATCATGGGAGTTCTCGAGAACTCCCATGATGTTGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACAACATCATGGGAGTTCTCGAGAACTCCCATGATGTTGTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000276174