Construct: shRNA TRCN0000149700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017645.3-2116s1c1
DNA Barcode:
GCCAGAATCATTACCTGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HAUS6 (54801)
Gene Description:
HAUS augmin like complex subunit 6
Transcript:
RefSeq NM_017645.3 (NON-CURRENT)
Match location:
Position 2116 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149700)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAATCATTACCTGTGTT
Hairpin Sequence:
5'-CCGG-GCCAGAATCATTACCTGTGTT-CTCGAG-AACACAGGTAATGATTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAATCATTACCTGTGTTCTCGAGAACACAGGTAATGATTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAATCATTACCTGTGTTCTCGAGAACACAGGTAATGATTCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000275877