Construct: shRNA TRCN0000149804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015115.1-1678s1c1
DNA Barcode:
GCATTCTTCTAGCCAGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCUN1D4 (23142)
Gene Description:
defective in cullin neddylation 1 domain containing 4
Transcript:
RefSeq NM_015115.1 (NON-CURRENT)
Match location:
Position 1678 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCTTCTAGCCAGTGATT
Hairpin Sequence:
5'-CCGG-GCATTCTTCTAGCCAGTGATT-CTCGAG-AATCACTGGCTAGAAGAATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCTTCTAGCCAGTGATTCTCGAGAATCACTGGCTAGAAGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCTTCTAGCCAGTGATTCTCGAGAATCACTGGCTAGAAGAATGC-3'

Other clones with same target sequence:

(none)