Construct: shRNA TRCN0000149831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173518.2-1910s1c1
DNA Barcode:
CTGCTTGATTGCTAGTTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCMDC2 (157777)
Gene Description:
minichromosome maintenance domain containing 2
Transcript:
RefSeq NM_173518.2 (NON-CURRENT)
Match location:
Position 1910 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTTGATTGCTAGTTGGTT
Hairpin Sequence:
5'-CCGG-CTGCTTGATTGCTAGTTGGTT-CTCGAG-AACCAACTAGCAATCAAGCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTTGATTGCTAGTTGGTTCTCGAGAACCAACTAGCAATCAAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTTGATTGCTAGTTGGTTCTCGAGAACCAACTAGCAATCAAGCAG-3'

Other clones with same target sequence:

(none)