Construct: shRNA TRCN0000149848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031246.1-718s1c1
DNA Barcode:
CAGGACCCTATGAATGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSG2 (5670)
Gene Description:
pregnancy specific beta-1-glycoprotein 2
Transcript:
RefSeq NM_031246.1 (NON-CURRENT)
Match location:
Position 718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGACCCTATGAATGTGAAA
Hairpin Sequence:
5'-CCGG-CAGGACCCTATGAATGTGAAA-CTCGAG-TTTCACATTCATAGGGTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGACCCTATGAATGTGAAACTCGAGTTTCACATTCATAGGGTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGACCCTATGAATGTGAAACTCGAGTTTCACATTCATAGGGTCCTG-3'

Other clones with same target sequence:

  1. TRCN0000162034
  2. TRCN0000153859