Construct: shRNA TRCN0000149861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002979.2-1295s1c1
DNA Barcode:
GCTCTGCAAGTGATGGATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCP2 (6342)
Gene Description:
sterol carrier protein 2
Transcript:
RefSeq NM_002979.2 (NON-CURRENT)
Match location:
Position 1295 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGCAAGTGATGGATTTA
Hairpin Sequence:
5'-CCGG-GCTCTGCAAGTGATGGATTTA-CTCGAG-TAAATCCATCACTTGCAGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGCAAGTGATGGATTTACTCGAGTAAATCCATCACTTGCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGCAAGTGATGGATTTACTCGAGTAAATCCATCACTTGCAGAGC-3'

Other clones with same target sequence:

(none)