Construct: shRNA TRCN0000149881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033285.2-4588s1c1
DNA Barcode:
GCCTTCTATCTTGGATGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53INP1 (94241)
Gene Description:
tumor protein p53 inducible nuclear protein 1
Transcript:
RefSeq NM_033285.2 (NON-CURRENT)
Match location:
Position 4588 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTATCTTGGATGGTTT
Hairpin Sequence:
5'-CCGG-GCCTTCTATCTTGGATGGTTT-CTCGAG-AAACCATCCAAGATAGAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTATCTTGGATGGTTTCTCGAGAAACCATCCAAGATAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTATCTTGGATGGTTTCTCGAGAAACCATCCAAGATAGAAGGC-3'

Other clones with same target sequence:

(none)