Construct: shRNA TRCN0000149894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152474.2-525s1c1
DNA Barcode:
GAACCTCAGGATACCGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C19orf18 (147685)
Gene Description:
chromosome 19 open reading frame 18
Transcript:
RefSeq NM_152474.2 (NON-CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTCAGGATACCGTTATT
Hairpin Sequence:
5'-CCGG-GAACCTCAGGATACCGTTATT-CTCGAG-AATAACGGTATCCTGAGGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTCAGGATACCGTTATTCTCGAGAATAACGGTATCCTGAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTCAGGATACCGTTATTCTCGAGAATAACGGTATCCTGAGGTTC-3'

Other clones with same target sequence:

(none)