Construct: shRNA TRCN0000149921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004537.3-572s1c1
DNA Barcode:
GCCAAGATTGAAGATGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAP1L1 (4673)
Gene Description:
nucleosome assembly protein 1 like 1
Transcript:
RefSeq NM_004537.3 (NON-CURRENT)
Match location:
Position 572 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000149921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGATTGAAGATGAGAAA
Hairpin Sequence:
5'-CCGG-GCCAAGATTGAAGATGAGAAA-CTCGAG-TTTCTCATCTTCAATCTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGATTGAAGATGAGAAACTCGAGTTTCTCATCTTCAATCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGATTGAAGATGAGAAACTCGAGTTTCTCATCTTCAATCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000280626