Construct: shRNA TRCN0000150012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152404.2-483s1c1
DNA Barcode:
CCCTTGTAAAGCTAATGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT3A1 (133688)
Gene Description:
UDP glycosyltransferase family 3 member A1
Transcript:
RefSeq NM_152404.2 (NON-CURRENT)
Match location:
Position 483 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGTAAAGCTAATGGAAA
Hairpin Sequence:
5'-CCGG-CCCTTGTAAAGCTAATGGAAA-CTCGAG-TTTCCATTAGCTTTACAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGTAAAGCTAATGGAAACTCGAGTTTCCATTAGCTTTACAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGTAAAGCTAATGGAAACTCGAGTTTCCATTAGCTTTACAAGGG-3'

Other clones with same target sequence:

(none)