Construct: shRNA TRCN0000150132

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014618.1-603s1c1
DNA Barcode:
GTCAAGGATTTACAACCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRINP1 (1620)
Gene Description:
BMP/retinoic acid inducible neural specific 1
Transcript:
RefSeq NM_014618.1 (NON-CURRENT)
Match location:
Position 603 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150132)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAAGGATTTACAACCAGAT
Hairpin Sequence:
5'-CCGG-GTCAAGGATTTACAACCAGAT-CTCGAG-ATCTGGTTGTAAATCCTTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAAGGATTTACAACCAGATCTCGAGATCTGGTTGTAAATCCTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAAGGATTTACAACCAGATCTCGAGATCTGGTTGTAAATCCTTGAC-3'

Other clones with same target sequence:

(none)