Construct: shRNA TRCN0000150174

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014933.2-3959s1c1
DNA Barcode:
GCTGATATACTCACCTTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC31A (22872)
Gene Description:
SEC31 homolog A, COPII coat complex component
Transcript:
RefSeq NM_014933.2 (NON-CURRENT)
Match location:
Position 3959 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150174)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATATACTCACCTTAGAA
Hairpin Sequence:
5'-CCGG-GCTGATATACTCACCTTAGAA-CTCGAG-TTCTAAGGTGAGTATATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATATACTCACCTTAGAACTCGAGTTCTAAGGTGAGTATATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATATACTCACCTTAGAACTCGAGTTCTAAGGTGAGTATATCAGC-3'

Other clones with same target sequence:

(none)