Construct: shRNA TRCN0000150257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015091.1-5108s1c1
DNA Barcode:
GCTGATATTGTTACGGAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOGARAM1 (23116)
Gene Description:
TOG array regulator of axonemal microtubules 1
Transcript:
RefSeq NM_015091.1 (NON-CURRENT)
Match location:
Position 5108 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATATTGTTACGGAACTT
Hairpin Sequence:
5'-CCGG-GCTGATATTGTTACGGAACTT-CTCGAG-AAGTTCCGTAACAATATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATATTGTTACGGAACTTCTCGAGAAGTTCCGTAACAATATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATATTGTTACGGAACTTCTCGAGAAGTTCCGTAACAATATCAGC-3'

Other clones with same target sequence:

(none)