Construct: shRNA TRCN0000150272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033394.1-6401s1c1
DNA Barcode:
GCAGAATATGAGAACACCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TANC1 (85461)
Gene Description:
tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Transcript:
RefSeq NM_033394.1 (NON-CURRENT)
Match location:
Position 6401 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATATGAGAACACCTAA
Hairpin Sequence:
5'-CCGG-GCAGAATATGAGAACACCTAA-CTCGAG-TTAGGTGTTCTCATATTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATATGAGAACACCTAACTCGAGTTAGGTGTTCTCATATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATATGAGAACACCTAACTCGAGTTAGGTGTTCTCATATTCTGC-3'

Other clones with same target sequence:

(none)