Construct: shRNA TRCN0000150291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030755.3-1148s1c1
DNA Barcode:
CGTGCCAAGCAATAAGATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMX1 (81542)
Gene Description:
thioredoxin related transmembrane protein 1
Transcript:
RefSeq NM_030755.3 (NON-CURRENT)
Match location:
Position 1148 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCCAAGCAATAAGATTTA
Hairpin Sequence:
5'-CCGG-CGTGCCAAGCAATAAGATTTA-CTCGAG-TAAATCTTATTGCTTGGCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCCAAGCAATAAGATTTACTCGAGTAAATCTTATTGCTTGGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCCAAGCAATAAGATTTACTCGAGTAAATCTTATTGCTTGGCACG-3'

Other clones with same target sequence:

  1. TRCN0000338653