Construct: shRNA TRCN0000150316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021255.1-2192s1c1
DNA Barcode:
CCAGAACTGTTTGGTTGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PELI2 (57161)
Gene Description:
pellino E3 ubiquitin protein ligase family member 2
Transcript:
RefSeq NM_021255.1 (NON-CURRENT)
Match location:
Position 2192 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAACTGTTTGGTTGATTA
Hairpin Sequence:
5'-CCGG-CCAGAACTGTTTGGTTGATTA-CTCGAG-TAATCAACCAAACAGTTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAACTGTTTGGTTGATTACTCGAGTAATCAACCAAACAGTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAACTGTTTGGTTGATTACTCGAGTAATCAACCAAACAGTTCTGG-3'

Other clones with same target sequence:

(none)