Construct: shRNA TRCN0000150325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172069.1-1776s1c1
DNA Barcode:
CCTTCAATATGGAGAGTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHH2 (130271)
Gene Description:
pleckstrin homology, MyTH4 and FERM domain containing H2
Transcript:
RefSeq NM_172069.1 (NON-CURRENT)
Match location:
Position 1776 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCAATATGGAGAGTGTTA
Hairpin Sequence:
5'-CCGG-CCTTCAATATGGAGAGTGTTA-CTCGAG-TAACACTCTCCATATTGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCAATATGGAGAGTGTTACTCGAGTAACACTCTCCATATTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCAATATGGAGAGTGTTACTCGAGTAACACTCTCCATATTGAAGG-3'

Other clones with same target sequence:

(none)