Construct: shRNA TRCN0000150339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022072.2-654s1c1
DNA Barcode:
CCACAGCCTTTGATAAATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSUN3 (63899)
Gene Description:
NOP2/Sun RNA methyltransferase 3
Transcript:
RefSeq NM_022072.2 (NON-CURRENT)
Match location:
Position 654 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGCCTTTGATAAATGTA
Hairpin Sequence:
5'-CCGG-CCACAGCCTTTGATAAATGTA-CTCGAG-TACATTTATCAAAGGCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGCCTTTGATAAATGTACTCGAGTACATTTATCAAAGGCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGCCTTTGATAAATGTACTCGAGTACATTTATCAAAGGCTGTGG-3'

Other clones with same target sequence:

(none)