Construct: shRNA TRCN0000150341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032819.3-2967s1c1
DNA Barcode:
CCATAAACTCTTCGGAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF341 (84905)
Gene Description:
zinc finger protein 341
Transcript:
RefSeq NM_032819.3 (NON-CURRENT)
Match location:
Position 2967 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAAACTCTTCGGAGTTTA
Hairpin Sequence:
5'-CCGG-CCATAAACTCTTCGGAGTTTA-CTCGAG-TAAACTCCGAAGAGTTTATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAAACTCTTCGGAGTTTACTCGAGTAAACTCCGAAGAGTTTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAAACTCTTCGGAGTTTACTCGAGTAAACTCCGAAGAGTTTATGG-3'

Other clones with same target sequence:

(none)