Construct: shRNA TRCN0000150568

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173855.2-372s1c1
DNA Barcode:
GTGATAAGAAATCGGGTTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MORN3 (283385)
Gene Description:
MORN repeat containing 3
Transcript:
RefSeq NM_173855.2 (NON-CURRENT)
Match location:
Position 372 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150568)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATAAGAAATCGGGTTATG
Hairpin Sequence:
5'-CCGG-GTGATAAGAAATCGGGTTATG-CTCGAG-CATAACCCGATTTCTTATCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATAAGAAATCGGGTTATGCTCGAGCATAACCCGATTTCTTATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATAAGAAATCGGGTTATGCTCGAGCATAACCCGATTTCTTATCAC-3'

Other clones with same target sequence:

(none)