Construct: shRNA TRCN0000150620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173514.1-1790s1c1
DNA Barcode:
GCTAACCTGATTGTTCAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC38A9 (153129)
Gene Description:
solute carrier family 38 member 9
Transcript:
RefSeq NM_173514.1 (NON-CURRENT)
Match location:
Position 1790 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACCTGATTGTTCAGTTT
Hairpin Sequence:
5'-CCGG-GCTAACCTGATTGTTCAGTTT-CTCGAG-AAACTGAACAATCAGGTTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACCTGATTGTTCAGTTTCTCGAGAAACTGAACAATCAGGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACCTGATTGTTCAGTTTCTCGAGAAACTGAACAATCAGGTTAGC-3'

Other clones with same target sequence:

(none)