Construct: shRNA TRCN0000150649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017921.1-3290s1c1
DNA Barcode:
GCACTATGAACAGAGAACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPLOC4 (55666)
Gene Description:
NPL4 homolog, ubiquitin recognition factor
Transcript:
RefSeq NM_017921.1 (NON-CURRENT)
Match location:
Position 3290 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTATGAACAGAGAACATT
Hairpin Sequence:
5'-CCGG-GCACTATGAACAGAGAACATT-CTCGAG-AATGTTCTCTGTTCATAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTATGAACAGAGAACATTCTCGAGAATGTTCTCTGTTCATAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTATGAACAGAGAACATTCTCGAGAATGTTCTCTGTTCATAGTGC-3'

Other clones with same target sequence:

(none)