Construct: shRNA TRCN0000150710

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005054.1-2718s1c1
DNA Barcode:
CCAGCATATAATTCCCAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGPD5 (84220)
Gene Description:
RANBP2 like and GRIP domain containing 5
Transcript:
RefSeq NM_005054.1 (NON-CURRENT)
Match location:
Position 2718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150710)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCATATAATTCCCAGTAT
Hairpin Sequence:
5'-CCGG-CCAGCATATAATTCCCAGTAT-CTCGAG-ATACTGGGAATTATATGCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCATATAATTCCCAGTATCTCGAGATACTGGGAATTATATGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCATATAATTCCCAGTATCTCGAGATACTGGGAATTATATGCTGG-3'

Other clones with same target sequence:

(none)