Construct: shRNA TRCN0000150739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014636.1-1875s1c1
DNA Barcode:
GCATTTGGATGATGCATGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGPS1 (9649)
Gene Description:
Ral GEF with PH domain and SH3 binding motif 1
Transcript:
RefSeq NM_014636.1 (NON-CURRENT)
Match location:
Position 1875 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTTGGATGATGCATGTAA
Hairpin Sequence:
5'-CCGG-GCATTTGGATGATGCATGTAA-CTCGAG-TTACATGCATCATCCAAATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTTGGATGATGCATGTAACTCGAGTTACATGCATCATCCAAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTTGGATGATGCATGTAACTCGAGTTACATGCATCATCCAAATGC-3'

Other clones with same target sequence:

(none)