Construct: shRNA TRCN0000150741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015555.1-3849s1c1
DNA Barcode:
GCTTTAATTAGAGCAGCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF451 (26036)
Gene Description:
zinc finger protein 451
Transcript:
RefSeq NM_015555.1 (NON-CURRENT)
Match location:
Position 3849 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAATTAGAGCAGCACAT
Hairpin Sequence:
5'-CCGG-GCTTTAATTAGAGCAGCACAT-CTCGAG-ATGTGCTGCTCTAATTAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAATTAGAGCAGCACATCTCGAGATGTGCTGCTCTAATTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAATTAGAGCAGCACATCTCGAGATGTGCTGCTCTAATTAAAGC-3'

Other clones with same target sequence:

(none)