Construct: shRNA TRCN0000150774

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001131.2-1200s1c1
DNA Barcode:
GCACAACTAACATCCAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRISP1 (167)
Gene Description:
cysteine rich secretory protein 1
Transcript:
RefSeq NM_001131.2 (NON-CURRENT)
Match location:
Position 1200 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150774)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAACTAACATCCAGTAAT
Hairpin Sequence:
5'-CCGG-GCACAACTAACATCCAGTAAT-CTCGAG-ATTACTGGATGTTAGTTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAACTAACATCCAGTAATCTCGAGATTACTGGATGTTAGTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAACTAACATCCAGTAATCTCGAGATTACTGGATGTTAGTTGTGC-3'

Other clones with same target sequence:

(none)