Construct: shRNA TRCN0000150779

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005363.2-626s1c1
DNA Barcode:
GTACTTCTTTCCTGTGATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEA6 (4105)
Gene Description:
MAGE family member A6
Transcript:
RefSeq NM_005363.2 (NON-CURRENT)
Match location:
Position 626 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150779)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACTTCTTTCCTGTGATCTT
Hairpin Sequence:
5'-CCGG-GTACTTCTTTCCTGTGATCTT-CTCGAG-AAGATCACAGGAAAGAAGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACTTCTTTCCTGTGATCTTCTCGAGAAGATCACAGGAAAGAAGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACTTCTTTCCTGTGATCTTCTCGAGAAGATCACAGGAAAGAAGTAC-3'

Other clones with same target sequence:

(none)