Construct: shRNA TRCN0000150813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012453.1-367s1c1
DNA Barcode:
GAACATATCTTGCATGGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBL2 (26608)
Gene Description:
transducin beta like 2
Transcript:
RefSeq NM_012453.1 (NON-CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACATATCTTGCATGGACTT
Hairpin Sequence:
5'-CCGG-GAACATATCTTGCATGGACTT-CTCGAG-AAGTCCATGCAAGATATGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACATATCTTGCATGGACTTCTCGAGAAGTCCATGCAAGATATGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACATATCTTGCATGGACTTCTCGAGAAGTCCATGCAAGATATGTTC-3'

Other clones with same target sequence:

(none)